#How to use blast from command-line, on Mac OS X.
Install blast
Double click the newly mounted ncbi-blast-2.2.18+ volume, double click on ncbi-blast-2.2.18+.pkg and follow the instructions in the installer. By default the BLAST+ applications are installed in /usr/local/ncbi/blast, overwriting its previous contents (an uninstaller is provided and it is recommended when upgrading a BLAST+ installation). Load more results. Apple Footer Apple Support. How To Install and Use NCBI Blast+ on Windows. This page is prepared for the people who want to use NCBI Blast+ on local machines, because we found most of the NCBI Blast+ information available on the Internet is out-dated or inaccurate. Blast: Contains functions for performing BLAST searches and resetting results. Interpro: It will run the InterProScan, reset the results or merge the GO results to the existing Annotation. Mapping: This function fetches GO terms associated to hit sequences obtained by BLAST. Annot: Includes di erent functions to obtain and modulate GO.
Download executables (binary) of blast-commands for Mac OS X.
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Install via
ncbi-blast-2.2.28+.dmg
Then, you can use folowing commands,
Next, download fasta-file to create database for blast.In this example, we use Swissprot.
Then, create databese for blast form this fasta-file.
Use blast from command-line
In this example, we will use
blastp
. blastp
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query: protein, database: protein.
User Manual Example
Use like following,
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Blast User Manual Mac Os X
Notice:For option -db, you have to write /path/to/db/<db-name>,e.g.,
Instruction Manual
Otherwise (setting just path to db directory /path/to/db/), you will get foloowing error.